Fusion user manual clontech

Clontech Laboratories, Inc. www.doorway.ru Protocol # PT 4 Version # PR74 30 Glutathione Resins User Manual The GST tag is specifically purified from the whole cell extract both as a fusion with DHFR and as a free protein. Whole cell extracts containing GST-DHFR (Panel A) . HQ Fusion. HQ Fusion User Manual – Version – For HQ Fusion machines with new features; HQ Fusion User Manual – Version – For HQ Fusion machines with new features; HQ Avanté. HQ Avanté User Manual version – For the HQ Avanté with . Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT–1 A Takara Bio Company Version No. 5 Matchmaker Gold Yeast Two-Hybrid System User Manual I. Introduction Protocol Overview continued Figure 2. Reporter gene constructs in Matchmaker yeast strains. In Y2HGold, the HIS3, ADE2, and MEL1/AUR1-C reporter genes are under.

CUP1 promoters driving expression of GST fusion proteins. pYEX-S1 (#) is used for expression and secretion of cloned proteins, including GST-tagged proteins. For ordering information on these products, please see Chapter XI of this Handbook or the CLONTECH Catalog. Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT A Takara Bio Company Version No. PR9Z 3 In-Fusion™ Advantage PCR Cloning Kit User Manual In-Fusion™ Advantage PCR Cloning Kits are designed to join multiple pieces of DNA that have 15 bases of homology at their linear ends. A typical use for this technology would be to clone. all other Clontech prelinearized In-Fusion Ready vectors if the gene of interest contains an initiating ATG. For additional information regarding In-Fusion cloning, see the In-Fusion HD Cloning Kit User Manual. B. Cloning Procedure 1. Combine the following in an eppendorf tube: 1 µl Linearized In-Fusion Ready Vector ( ng/μl).

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